全文获取类型
收费全文 | 2796篇 |
免费 | 116篇 |
国内免费 | 30篇 |
专业分类
耳鼻咽喉 | 8篇 |
儿科学 | 113篇 |
妇产科学 | 24篇 |
基础医学 | 557篇 |
口腔科学 | 100篇 |
临床医学 | 281篇 |
内科学 | 559篇 |
皮肤病学 | 116篇 |
神经病学 | 177篇 |
特种医学 | 327篇 |
外科学 | 213篇 |
综合类 | 48篇 |
预防医学 | 88篇 |
眼科学 | 98篇 |
药学 | 174篇 |
中国医学 | 4篇 |
肿瘤学 | 55篇 |
出版年
2016年 | 29篇 |
2015年 | 32篇 |
2014年 | 33篇 |
2013年 | 95篇 |
2012年 | 63篇 |
2011年 | 44篇 |
2010年 | 65篇 |
2009年 | 51篇 |
2008年 | 52篇 |
2007年 | 66篇 |
2006年 | 82篇 |
2005年 | 66篇 |
2004年 | 49篇 |
2003年 | 49篇 |
2002年 | 47篇 |
2001年 | 64篇 |
2000年 | 60篇 |
1999年 | 50篇 |
1998年 | 90篇 |
1997年 | 99篇 |
1996年 | 105篇 |
1995年 | 76篇 |
1994年 | 78篇 |
1993年 | 77篇 |
1992年 | 44篇 |
1991年 | 42篇 |
1990年 | 46篇 |
1989年 | 77篇 |
1988年 | 63篇 |
1987年 | 54篇 |
1986年 | 58篇 |
1985年 | 47篇 |
1984年 | 57篇 |
1983年 | 33篇 |
1982年 | 34篇 |
1981年 | 40篇 |
1980年 | 50篇 |
1978年 | 29篇 |
1977年 | 36篇 |
1976年 | 38篇 |
1975年 | 38篇 |
1972年 | 29篇 |
1971年 | 25篇 |
1970年 | 27篇 |
1965年 | 50篇 |
1964年 | 53篇 |
1963年 | 48篇 |
1962年 | 42篇 |
1961年 | 40篇 |
1960年 | 66篇 |
排序方式: 共有2942条查询结果,搜索用时 15 毫秒
81.
82.
力竭运动大鼠心室肌蛋白质组表达特征 总被引:3,自引:0,他引:3
目的:采用蛋白质组学技术,建立安静和递增运动负荷训练后力竭大鼠心室肌蛋白质组的差异性表达谱,初步筛选出心室肌对力竭运动产生反应的目标蛋白质。方法:实验于2007-03在湖南师范大学生命科学学院蛋白质化学与蛋白质组学国家教育部重点实验室和省级运动人体科学实验室完成。①实验分组:10只SD雄性大鼠随机分为对照组和运动组,每组5只。②实验方法:运动组经过7周的大强度递增运动负荷训练后(最后一次力竭),对两组心室肌组织的全蛋白进行双向凝胶电泳分离。结果:经图像分析,在运动组的电泳图谱上共展现蛋白质点(338±17)个,对照组展现蛋白质点(352±17)个。运动后差异表达的蛋白质点共有99个。对其中差异表达的9个蛋白质点进行质谱鉴定,共鉴定出7个蛋白质,Stress-70protein,NADH-ubiquinone oxidoreductase Mr75000subnunit,Long-chain specific acyl-CoA dehydrogenase,Tropomyosin-1alphachain在运动后"缺失",Nitrilase family,member2在运动后表达上调在5倍以上,一个相对分子质量为21000的未知蛋白在运动后表达下调在5倍以上,另外有两个点经鉴定均为Myosin-6,在运动后表达量相反。这些蛋白质属于收缩蛋白、能量代谢酶、分子伴侣等。结论:递增运动负荷训练后力竭时,大鼠心室肌蛋白质组明显地发生了反应。运动后"缺失"和下调的蛋白质点与心肌收缩的调控和能量代谢的方式转变以及细胞的应激反应有关,其中,成功筛选出6种在运动医学领域尚未涉足的、具有运动应激特点的目标蛋白质。 相似文献
83.
H. WESTON MOSES BERNARD F. SCHREINER JR. RICHARD W. HYDE MICHAEL C. KALLAY 《Pacing and clinical electrophysiology : PACE》1982,5(6):826-828
A young woman with idiopathic pulmonary hemosiderosis (IPH) and advanced heart block below the atrioventricular node requiring pacemaker therapy is discussed. Thirteen years after the onset of IPH she is free of pulmonary symptoms despite having never received steroid or antimetabolite therapy. 相似文献
84.
85.
86.
Wolfgang H?rtig Andreas Reichenbach Cornelia Voigt Johannes Boltze Larysa Bulavina Martin U. Schuhmann Johannes Seeger Gerald F. Schusser Christiane Freytag Jens Grosche 《Journal of chemical neuroanatomy》2009,37(2):128-138
The simultaneous detection of glia, vessels and neurons facilitates insights into the complex chemoarchitecture of the central nervous system. Here, we present a simple, robust and versatile approach for the carbocyanine triple fluorescence labelling of neuronal, vascular and glial markers.The usefulness of this procedure is shown for rat brain tissue under physiological conditions, after traumatic brain injury caused by controlled cortical impact injury, and after stroke following middle cerebral artery occlusion. Moreover, the versatility of the method is verified by its application to sections from old triple transgenic mice with age-dependent β-amyloidosis and tau hyperphosphorylation in the hippocampus, modelling neuropathological alterations in Alzheimer‘s disease. To exemplify the usefulness of the approach for analysis of the enteric nervous system, it was applied to whole mounts from the horse intestine.The biotinylated lectin from potato (Solanum tuberosum) is presented as an excellent tool to detect both vessels and microglia. Furthermore, this lectin revealed macrophages after experimental insults, and senile plaques in aged triple transgenic mice. A large portion of astroglia was demonstrated by immunolabelling of glial fibrillary acidic protein. Neurons were detected by monoclonal antibodies directed against neuronal nuclei and, in horse tissues, mouse-anti-HuC/D recognizing a conserved nuclear protein. Confocal laser-scanning microscopy elucidated spatial relationships of the relevant markers and their pathological alterations after experimental insults and in transgenic mice with Alzheimer-like lesions. 相似文献
87.
88.
DJ Crofts VJ-M Michel AS Rigby MS Tanner DMB Hall JR Bonham 《Acta paediatrica (Oslo, Norway : 1992)》1999,88(9):969-974
Jaundice persisting beyond the first 2 wk of life is often regarded as an indication for investigation to exclude cholestatic liver disease. Most babies with prolonged jaundice have breast milk-related jaundice, which is a benign condition. Cholestatic liver disease is usually accompanied by pale stools and yellow or orange urine. A community programme was established to ascertain the incidence of prolonged jaundice and determine whether abnormal stool and urine colour could be used to assist primary care staff in referral decisions. Data were collected on normal stool and urine colour and used to devise a colour chart and information sheet for parents. Babies with prolonged jaundice were identified and referred for investigation. In all, 3661 babies were recruited into the study, of which 127 were jaundiced at 28 d of age. Of these, 125 were breastfed. The incidence of jaundice in breastfed babies at 28 d was 9.2% (95% CI 7.8%-11.0%) Abnormal liver function tests (LFTs) were common, but no baby had abnormal stool or urine colour and none was found to have liver disease. Jaundiced breastfed babies who are well are unlikely to have serious disease. Elevated LFTs are compatible with a diagnosis of breast milk-related jaundice. Prolonged jaundice in bottle-fed babies, and persistent pallor of stools or yellow/orange urine, are rare and merit immediate referral. Parents and professionals can be advised to report pale stools without generating a large number of unnecessary referrals. Further work is needed to determine whether a colour chart reduces the mean age of referral and treatment of infants with cholestatic liver disease. 相似文献
89.
Ziel. Ziel unserer Untersuchungen war es, die diagnostische Wertigkeit einer neuen, r?umlich hochaufgel?sten MR-Venographietechnik bei Patienten mit zerebralen arterioven?sen Malformationen (AVM) zu untersuchen. 相似文献
90.